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The combination of HPLC-MS is oriented in the direction of the particular detection and prospective identification of substances during the presence of other chemical compounds. Even so, it's hard to interface the liquid chromatography to a mass-spectrometer, since the many solvents must be removed very first. The widespread used interface contains
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The cell phase carries the sample factors through the column, exactly where they communicate with the stationary section to various levels. This interaction establishes just how long Every element spends in the column, leading to their separation.. HPLC separation of a mix of flavonoids with UV/Vis detection at 360 nm and, in the inset, at 260 nm.